Systemic administration of an RNA binding and cell-penetrating antibody targets therapeutic RNA to multiple mouse models of cancer.

Quijano E, Martinez-Saucedo D, Ianniello Z, Pinto-Medici N, Rackear M, Chen H, Lola-Pereira L, Liu Y, Hegan D, Shan X, Tseng R, Yugawa D, Chowdhury S, Khang M, Singh JP, Abdullah R, Azhir P, Kashima S, Woods WS, Gosstola N, Turner BC, Squinto S, Ludwig DL, Bindra RS, Robert ME, Braun DA, Perez Pinera P, Saltzman WM, Escobar-Hoyos LF, Glazer PM.

Sci Transl Med. 2025 Jul 16;17(807):eadk1868.

Implementation of an Undergraduate Laboratory-Based Mammalian Genome Editing Course.

Guerra I, Jensen K, Perez-Pinera P.

CRISPR J. 2025 Oct;8(5):366-374.

SPLICER: a highly efficient base editing toolbox that enables in vivo therapeutic exon skipping.

Miskalis A, Shirguppe S, Winter J, Elias G, Swami D, Nambiar A, Stilger M, Woods WS, Gosstola N, Gapinske M, Zeballos A, Moore H, Maslov S, Gaj T, Perez-Pinera P.

Nat Commun. 2024 Nov 28;15(1):10354.

Chromatin-associated lncRNA-splicing factor condensates regulate hypoxia responsive RNA processing of genes pre-positioned near nuclear speckles.

Song YJ, Shinn MK, Bangru S, Wang Y, Sun Q, Hao Q, Chaturvedi P, Freier SM, Perez-Pinera P, Nelson ER, Belmont AS, Guttman M, Prasanth SG, Kalsotra A, Pappu RV, Prasanth KV.

bioRxiv [Preprint]. 2024 Oct 31:2024.10.31.621310.

In vivo CRISPR base editing for treatment of Huntington's disease.

Shirguppe S, Gapinske M, Swami D, Gosstola N, Acharya P, Miskalis A, Joulani D, Szkwarek MG, Bhattacharjee A, Elias G, Stilger M, Winter J, Woods WS, Anand D, Lim CKW, Gaj T, Perez-Pinera P.

bioRxiv [Preprint]. 2024 Jul 6:2024.07.05.602282.

Protein Engineering Technologies for Development of Next-Generation Genome Editors.

Winter J, Shirguppe S, Perez-Pinera P.

Curr Opin Biomed Eng. 2023 Dec;28:100514.

Targeting Duchenne muscular dystrophy by skipping DMD exon 45 with base editors.

Gapinske M, Winter J, Swami D, Gapinske L, Woods WS, Shirguppe S, Miskalis A, Busza A, Joulani D, Kao CJ, Kostan K, Bigot A, Bashir R, Perez-Pinera P.

Mol Ther Nucleic Acids. 2023 Jul 27;33:572-586.

Chromatin structure and context-dependent sequence features control prime editing efficiency.

Kim S, Yuan JB, Woods WS, Newton DA, Perez-Pinera P, Song JS.

Front Genet. 2023 Jun 29;14:1222112.

Delivering Base Editors In Vivo by Adeno-Associated Virus Vectors.

Lim CKW, Miskalis AJ, Perez-Pinera P, Gaj T.

Methods Mol Biol. 2023;2606:135-158.

Delineating cooperative effects of Notch and biomechanical signals on patterned liver differentiation.

Jain I, Berg IC, Acharya A, Blaauw M, Gosstola N, Perez-Pinera P, Underhill GH.

Commun Biol. 2022 Oct 7;5(1):1073.

CRISPR base editing of cis-regulatory elements enables the perturbation of neurodegeneration-linked genes.

Lim CKW, McCallister TX, Saporito-Magriña C, McPheron GD, Krishnan R, Zeballos C MA, Powell JE, Clark LV, Perez-Pinera P, Gaj T.

Mol Ther. 2022 Dec 7;30(12):3619-3631.

Development and Implementation of a Remote Enzyme Kinetics Laboratory Exercise.

Bhattacharjee A, Johnson E, Perez-Pinera P, Jensen K.

J Microbiol Biol Educ. 2022 Mar 28;23(1):e00286-21.

Remote Laboratory Exercise to Develop Micropipetting Skills.

Jawad MN, Bhattacharjee A, Lehmann R, Busza A, Perez-Pinera P, Jensen K.

J Microbiol Biol Educ. 2021 Mar 31;22(1):22.1.27.

ABC-GWAS: Functional Annotation of Estrogen Receptor-Positive Breast Cancer Genetic Variants.

Manjunath M, Zhang Y, Zhang S, Roy S, Perez-Pinera P, Song JS.

Front Genet. 2020 Jul 20;11:730.

Optimizing Quantum Dot Probe Size for Single-Receptor Imaging.

Le P, Vaidya R, Smith LD, Han Z, Zahid MU, Winter J, Sarkar S, Chung HJ, Perez-Pinera P, Selvin PR, Smith AM.

ACS Nano. 2020 Jul 28;14(7):8343-8358.

Epigenetic engineering of yeast reveals dynamic molecular adaptation to methylation stress and genetic modulators of specific DNMT3 family members.

Finnegan AI, Kim S, Jin H, Gapinske M, Woods WS, Perez-Pinera P, Song JS.

Nucleic Acids Res. 2020 May 7;48(8):4081-4099.

Treatment of a Mouse Model of ALS by In Vivo Base Editing.

Lim CKW, Gapinske M, Brooks AK, Woods WS, Powell JE, Zeballos C MA, Winter J, Perez-Pinera P, Gaj T.

Mol Ther. 2020 Apr 8;28(4):1177-1189.

Targeted exon skipping with AAV-mediated split adenine base editors.

Winter J, Luu A, Gapinske M, Manandhar S, Shirguppe S, Woods WS, Song JS, Perez-Pinera P.

Cell Discov. 2019 Aug 20;5:41.

Directed Evolution of CRISPR-Cas9 Base Editors.

Winter J, Perez-Pinera P.

Trends Biotechnol. 2019 Nov;37(11):1151-1153.

Multiplexed and tunable transcriptional activation by promoter insertion using nuclease-assisted vector integration.

Brown A, Winter J, Gapinske M, Tague N, Woods WS, Perez-Pinera P.

Nucleic Acids Res. 2019 Jul 9;47(12):e67.

The continuously evolving CRISPR barcoding toolbox.

Gaj T, Perez-Pinera P.

Genome Biol. 2018 Sep 25;19(1):143.

Disruption of the β1L Isoform of GABP Reverses Glioblastoma Replicative Immortality in a TERT Promoter Mutation-Dependent Manner.

Mancini A, Xavier-Magalhães A, Woods WS, Nguyen KT, Amen AM, Hayes JL, Fellmann C, Gapinske M, McKinney AM, Hong C, Jones LE, Walsh KM, Bell RJA, Doudna JA, Costa BM, Song JS, Perez-Pinera P, Costello JF.

Cancer Cell. 2018 Sep 10;34(3):513-528.e8.

CRISPR-SKIP: programmable gene splicing with single base editors.

Gapinske M, Luu A, Winter J, Woods WS, Kostan KA, Shiva N, Song JS, Perez-Pinera P.

Genome Biol. 2018 Aug 15;19(1):107.

Targeted Gene Knock Out Using Nuclease-Assisted Vector Integration: Hemi- and Homozygous Deletion of JAG1.

Gapinske M, Tague N, Winter J, Underhill GH, Perez-Pinera P.

Methods Mol Biol. 2018;1772:233-248.

Versatile and on-demand biologics co-production in yeast.

Cao J, Perez-Pinera P, Lowenhaupt K, Wu MR, Purcell O, de la Fuente-Nunez C, Lu TK.

Nat Commun. 2018 Jan 8;9(1):77.

Production of Functional Anti-Ebola Antibodies in Pichia pastoris.

Purcell O, Opdensteinen P, Chen W, Lowenhaupt K, Brown A, Hermann M, Cao J, Tenhaef N, Kallweit E, Kastilan R, Sinskey AJ, Perez-Pinera P, Buyel JF, Lu TK.

ACS Synth Biol. 2017 Dec 15;6(12):2183-2190.

Mammalian Synthetic Biology: Engineering Biological Systems.

Black JB, Perez-Pinera P, Gersbach CA.

Annu Rev Biomed Eng. 2017 Jun 21;19:249-277.

Prosurvival long noncoding RNA PINCR regulates a subset of p53 targets in human colorectal cancer cells by binding to Matrin 3.

Chaudhary R, Gryder B, Woods WS, Subramanian M, Jones MF, Li XL, Jenkins LM, Shabalina SA, Mo M, Dasso M, Yang Y, Wakefield LM, Zhu Y, Frier SM, Moriarity BS, Prasanth KV, Perez-Pinera P, Lal A.

Elife. 2017 Jun 5;6:e23244.

Design and integration of a problem-based biofabrication course into an undergraduate biomedical engineering curriculum.

Raman R, Mitchell M, Perez-Pinera P, Bashir R, DeStefano L.

J Biol Eng. 2016 Sep 21;10:10.

Targeted Gene Activation Using RNA-Guided Nucleases.

Brown A, Woods WS, Perez-Pinera P.

Methods Mol Biol. 2017;1468:235-50.

Biomedical applications of gene editing.

Perez-Pinera P, Chen ZY.

Hum Genet. 2016 Sep;135(9):967-9.

Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care.

Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, Ram R, Lu TK.

Nat Commun. 2016 Jul 29;7:12211.

Multiplexed Targeted Genome Engineering Using a Universal Nuclease-Assisted Vector Integration System.

Brown A, Woods WS, Perez-Pinera P.

ACS Synth Biol. 2016 Jul 15;5(7):582-8.

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